medium 200 supplemented with large vessel endothelial supplement (lves)  (Lonza)

Journal: Scientific Reports

Article Title: Potential use of superparamagnetic iron oxide nanoparticles for in vitro and in vivo bioimaging of human myoblasts

doi: 10.1038/s41598-018-22018-0

Figure Lengend Snippet: HUVEC angiogenesis test. To evaluate the pro-angiogenic properties of secreted proteins in the supernatants collected from myoblasts under study, the tested samples were transferred onto prepared HUVEC cells. Supernatants were collected from: WT – non transfected myoblasts; p-TRUF-22 – mock transfected myoblasts; FGF-4/VEGF – myoblasts transfected with complete bicistronic plasmid; w/o SPIONs – myoblasts non-labeled with obtained SPIONs; SPIONs – myoblast labeled with obtained SPIONs (0.025 mg/µL medium). Negative controls: DMEM and fresh myoblast medium. Positive control: medium 200 (with Large Vessel Endothelial Supplement). Capillaries were stained with calcein. Scale bars indicate 500 µm.

Article Snippet: HUVECs were cultured (no further than to 4th passage) to reach about 80–90% confluency using Medium 200 supplemented with Large Vessel Endothelial Cells Supplement - LVES (Gibco, Carlsbad, USA).

Techniques: Transfection, Plasmid Preparation, Labeling, Positive Control, Staining